Objective. To compare the effect of power-assisted, syringe and traditional low and high negative pressure liposuction on cultural properties of adipose-derived stem cells (ADSC).

Material and methods. The study included 36 women without chronic diseases aged 18-40 years and body mass index 20.0-30.0 kg/m2. All women underwent adipose tissue harvesting within 4 areas (bilateral lower and upper posterolateral lumbar regions). Only one of four methods was used in each area: 1) traditional suction assisted low negative pressure liposuction (–250 mm Hg) (low-SAL); 2) traditional suction assisted high negative pressure liposuction (–750 mm Hg) (high-SAL); 3) syringe liposuction (SYR); 4) power assisted liposuction (PAL). Lipoaspirate samples were subjected to enzymatic preparation and cultured on nutrient media twice by 7 days. Then, we assessed the number of ADSC and their viability and performed immunophenotyping.

Results. The number of ADSC varied within a wide range (from 0.098·106/ml to 1.134·106/ml). There were no significant between-group differences with the exception of SYR and high-SAL groups. ADSC viability was high after cultivation (up to 90%) regardless liposuction technique. Regardless liposuction technique, ADSC intensively expressed CD13, CD29, CD44, CD73, CD90 and CD105 by the second passage and almost did not express markers of hematopoietic cells (CD31, CD34, CD45).

Conclusion. The choice of liposuction method has no effect on cultural properties of ADSC.

Keywords. liposuction, lipofilling, adipose-derived stem cells